The issue of improving hemorrhoid treatment outcomes in outpatient practice is one of the most pressing in the daily work of coloproctologists today. Advances in the treatment of hemorrhoidal disease, the most common pathological condition in general proctology, in recent decades have been associated not only with the adoption of minimally invasive surgical techniques but also with the enhancement of pharmacological therapy. A key component of systemic pharmacotherapy for chronic and acute hemorrhoids is the use of venotonic drugs, including flavonoids which are included in the clinical guidelines. Therefore, a comprehensive and reliable evaluation of the clinical efficacy of diosmin is an important area of research, especially in cases of acute hemorrhoids.

Post-thrombotic syndrome (PTS) has been highly prevalent, over 50% of the patients develop PTS following lower extremity acute deep vein thrombosis (DVT). Venoactive compounds (VACs) have been recommended for decades for patients with chronic venous insufficiency (CVI), including PTS. The objective of our study was to perform a systematic review to determine the quality of evidence on the utility of VACs for both prevention and treatment of PTS.

This research investigated the potential of zein-sodium caseinate-diosmin nanoparticles (ZCD-NPs) as an anti-cancer agent against the A2780 cell line. Dynamic light scattering (DLS) analysis showed that ZCD-NPs have an average size of 265.30 nm with a polydispersity index of 0.21, indicating good uniformity suitable for pharmaceutical applications. Fourier transform infrared spectroscopy (FTIR) confirmed the successful incorporation of diosmin into the NPs and highlighted the interactions between the components. Field emission scanning electron microscopy (FESEM) images showed spherical NPs with smooth surfaces, suggesting stability and high production quality. Encapsulation efficiency was remarkably high, at 93.45%. Cytotoxicity assays showed a dose-dependent effect of ZCD-NPs, with A2780 cells showing significant sensitivity compared to normal HDF cells, indicating selective targeting of cancer cells. Flow cytometry analysis confirmed that ZCD-NPs induced apoptosis and necrosis in A2780 cells, as evidenced by increased expression of apoptotic genes such as p53 and caspases 8 and 9. In addition, ZCD-NPs exhibited potent antioxidant activity, effectively scavenging free radicals. These results suggest that ZCD-NPs have promising properties for targeted cancer therapy and antioxidant applications, which warrant further exploration in clinical settings.

The European sweet cherry (Prunus avium) is highly valued for its superior quality, delectable taste, and robust stress resistance, leading to its extensive cultivation in the world. However, the previous incomplete genome assemblies have impeded its evolution and genetic regulation studies. In this study, we generated a Telomere-to-Telomere gap-free genome assembly of P. avium cv. Tieton, using advanced sequencing technologies. The assembled genome comprises eight pseudochromosomes with a genome size of 342.23 Mb and a contig N50 of 40.66 Mb. Comparative genomic analysis identified several unique stress resistance-related genes, possibly associated with the species' environmental adaptation. The integrative analyses of genomics, transcriptomes and metabolomes identified some key structural genes and metabolites crucial to flavonoid biosynthesis of sweet cherry. Our analyses revealed that 85 flavonoid metabolites, which are highly differentially accumulated among five tissues (flesh, stem, leaf, bud, and seed) of cherry. Interestingly, eight abundant flavonoids (Narcissoside, Typhaneoside, Myricetin 3-0-galactoside, Diosmin, Neohesperidin, Liquiritin apioside, 5,6,7-Trimethoxyflavone and Oroxin B) were highly accumulated in cherry flesh tissues. The gene-metabolite correlation analysis revealed that seven genes (HTC8, HTC6, CYP75B1_9, CYP75B1_10, 4CL1, DFR1, and FLS1) significantly regulated flavonoid accumulation in cherry flesh. Additionally, some structural genes (4CL6, PAL3, CYP75A2, F3H1, CYP75B1_8, and CYP75B1_10) were identified in the flavonoid biosynthetic pathway and were highly expressed, aligning with high flavonoid metabolite content in cherry flesh. These identified genes and metabolites are likely pivotal in conferring sweet cherry's stress resistance and high-quality traits. These findings offer deep insights into the mechanisms of genomic evolution and flavonoid biosynthesis, which also lay a solid foundation for further function genomics studies and breeding improvement in cherry.

Amikacin (AMC), an aminoglycoside antibiotic known for its rapid and potent bactericidal activity, is also associated with nephrotoxicity. Diosmin and perindopril have been reported to improve renal function and hold promise as therapeutic agents for preventing drug-induced nephrotoxicity. This study aimed to investigate the protective effect of Diosmin and perindopril, either alone or in combination, against renal damage induced by AMC toxicity and to elucidate the underlying mechanisms.

In this study, we investigated the inhibitory potential of 60 flavonoids from six distinct subgroups on the programmed cell death ligand 1 (PD-L1) dimer through molecular docking and dynamics simulations. Using AutoDock Vina for docking, the binding poses and affinities were evaluated, revealing an average binding affinity of -8.5 kcal/mol for the flavonoids. Among them, ginkgetin exhibited the highest binding free energy of -46.73 kcal/mol, indicating a strong interaction with PD-L1, while diosmin followed closely, with -44.96 kcal/mol. Molecular dynamics simulations were used to further elucidate the dynamic interactions and stability of the flavonoid-PD-L1 complexes, with the analyses showing minimal root mean square deviation (RMSD) and favorable root mean square fluctuation (RMSF) profiles for several compounds, particularly formononetin, idaein, and neohesperidin. Additionally, contact number and hydrogen bond analyses were performed, which highlighted ginkgetin and diosmin as key flavonoids with significant binding interactions, evidenced by their stable conformations and robust molecular interactions throughout the simulations. Ultimately, a cell-based assay confirmed their ability to inhibit the proliferation of cancer cells. These results, validated through cell-based assays, indicate that the strategy of identifying natural compounds with anticancer activity using computational modeling is highly effective.

Salmonella Typhimurium is an invasive intracellular pathogen that employs various factors for its survival within host cells. To mitigate S. Typhimurium survival, it is crucial to identify factors that influence bacterial survival and to develop drugs that inhibit these factors. In this study, we investigated the effects of nafcillin and diosmin, both of which have been identified as inhibitors of Lon protease, on the intracellular survival of S. Typhimurium and its survival under various stress conditions. Additionally, we examined the expression of genes associated with the type II toxin-antitoxin system to enhance our understanding of the impact of these systems on the bacterium's survival. Our findings indicate that while nafcillin and diosmin did not affect S. Typhimurium attachment, they significantly reduced bacterial intracellular survival, particularly in Hep2 cells after 16 h. These inhibitors were also effective in decreasing bacterial survival under oxidative and acidic stress conditions. Furthermore, gene expression analysis revealed that although there were variations in the expression of TA system genes in S. Typhimurium across different cell lines, the relEB system emerged as the most effective among those studied, exhibiting the highest increase in expression. This study highlights the efficacy of nafcillin and diosmin in reducing the intracellular survival of S. Typhimurium as well as its survival under stress conditions. These findings suggest potential new strategies for developing therapies aimed at preventing S. Typhimurium infections.

Cardiotoxicity is doxorubicin's primary side effect. Its cardiac toxicity has been attributed to the generation of free radicals. The present work was designed to understand the potential underlying pathways behind the cardioprotective action of diosmin (Dio) and Dio-loaded chitosan nanoparticles (DCNPs) against doxorubicin (Dox)-mediated cardiotoxicity. Male rats were allocated into five groups: control, Dio (100 mg/kg), Dox (12 mg/kg), Dio + Dox (100 mg/kg + 12 mg/kg), and DCNPs+Dox (100 mg/kg DCNPs/orally+12 mg/kg Dox/IP). Notably, in response to Dox, a significant increase of cardiac biomarkers with a decrease in Na/K-ATPase activity was detected. The cardiac inflammatory and pro-apoptotic protein levels were elevated with decreased cardiac interleukin-10 and Bcl-2 levels when the rats were subjected to Dox. Also, the cardiac expression of the fibrotic marker MMP-9 was increased. Moreover, Dox raised malondialdehyde and nitric oxide levels, accompanied by minimizing antioxidant status. Also, Dox-treated rats showed cardiac histopathological impairment compared to the control. The oral administration of Dio or DCNPs enhanced the activity of antioxidant enzymes and diminished inflammatory cytokines and apoptotic markers in the Dox-exposed rats. In summary, these findings indicate that DCNPs exhibit significant cardioprotective effectiveness against Dox-mediated toxicity by suppressing various mechanisms, such as redox status, the NF-κB pathway, and apoptosis.

, a plant species from the Lamiaceae family, is endemic to the Nabatian region, which spans southern Jordan and northwestern Saudi Arabia. It is renowned for its traditional uses and rich phytochemical profile. This study aims to examine the phytochemical composition of and evaluate its biological activities, including antioxidant capacity, cytotoxic effects on cancer cell lines, and enzyme inhibition relevant to diabetes and neurodegenerative diseases. The essential oil (EO) and methanol extract of were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS) and High-Performance Liquid Chromatography (HPLC). Antioxidant activity was assessed using the DPPH radical scavenging assay. Cytotoxicity was evaluated against MDA-MB231 and LNCaP cancer cell lines using the MTT assay. Enzyme inhibition assays were conducted to determine the inhibitory effects on α-amylase, α-glucosidase, and butyrylcholinesterase. GC-MS analysis revealed thymol (82.30%) as the major component of the essential oil, while HPLC identified significant phenolic compounds in the methanol extract, including diosmin (118.75 mg/g) and hesperidin (22.18 mg/g). The DPPH assay demonstrated strong antioxidant activity, with the methanol extract showing an IC50 of 11.97 μg/mL for α-amylase and 31.99 μg/mL for α-glucosidase, indicating notable antidiabetic potential. Cytotoxicity tests revealed significant antiproliferative effects against both cancer cell lines, with lower IC50 values compared to standard treatments. exhibits substantial antioxidant, cytotoxic, and enzyme inhibition activities, supporting its traditional medicinal uses. These findings provide a scientific basis for further research into its bioactive compounds and potential applications in modern pharmacology, particularly in developing natural therapeutic agents for oxidative stress-related diseases and cancer.

Chronic kidney disease (CKD) is an overriding concern for many researchers and physicians as it causes unspeakable suffering and anguish among patients. Renal fibrosis is the hallmark of end-stage kidney disease (ESKD), which can progress to death. The super-fuels for renal fibrosis are oxidative stress and inflammation. It occurs almost without exception in all CKD patients, but how it develops is still ambiguous. Flavonoids demonstrate tremendous fighting power against inflammation and oxidative stress in many diseases. NF-κB suppression has been deeply investigated in renal fibrosis, with phenomenally good outcomes. This review shed light on flavonoids' ability to beat renal fibrosis associated with NF-κB activation. Flavonoids such as apigenin, baicalin, diosmin, epigallocatechin-3-gallate, genistein, isoliquiritigenin, naringin, puerarin, quercetin, silibinin, wogonin, biochanin A, and cardamonin exhibit promising antifibrotic effects through targeting NF-κB. Moreover, in silico studies showed that flavonoids displayed an outstanding inhibitory effect on NF-κB based on the energy of binding. They are well-fitted to the binding pocket of the target protein by forming hydrogen bonds, hydrophobic, and ionic interactions with the key amino acid residues. The outcomes revealed that silibinin, baicalin, and baicalein are the most powerful NF-κB inhibitors. In conclusion, through suppressing NF-κB signal, flavonoids display a dynamite performance in combating CKD and renal fibrosis.