Associations of pesticide exposures during preconception with stillbirth have not been well explored. We linked Arizona pesticide use records with birth certificates from 2006 to 2020 and estimated associations of living within 500 m of any pyrethroid, organophosphate (OP), or carbamate pesticide applications during a 90-day preconception window or the first trimester, with stillbirth. We considered a binary measure of exposure (any exposure), as well as log-pounds and log-acres applied within 500 m, in a negative control exposure framework with log-binomial regression. We included 1 237 750 births, 2290 stillbirths, and 27 pesticides. During preconception, any exposure to pesticides was associated with stillbirth, including cyfluthrin (risk ratio [RR] = 1.97; 95% CI, 1.17-3.32); zeta-cypermethrin (RR = 1.81; 95% CI, 1.20-2.74); OPs as a class (RR = 1.60; 95% CI, 1.16-2.19); malathion (RR = 2.02; 95% CI, 1.26-3.24); carbaryl (RR = 6.39; 95% CI, 2.07-19.74); and propamocarb hydrochloride (RR = 7.72; 95% CI, 1.10-54.20). During the first trimester, fenpropathrin (RR = 4.36; 95% CI, 1.09-17.50); permethrin (RR = 1.57; 95% CI, 1.02-2.42); OPs as a class (RR = 1.50; 95% CI, 1.11-2.01); acephate (RR = 2.31; 95% CI, 1.22-4.40); and formetanate hydrochloride (RR = 7.22; 95% CI, 1.03-50.58) were associated with stillbirth. Interpretations were consistent when using continuous measures of pounds or acres of exposure. Pesticide exposures during preconception and first trimester may be associated with stillbirth. This article is part of a Special Collection on Environmental Epidemiology.

Cabbage maggot (CM) (Delia radicum L.) is a devastating pest of Brassicaceae crops throughout the world, including the Willamette Valley in western Oregon, USA. Chemical control methods for this pest are limited, with reduction or elimination of chlorpyrifos tolerances and expensive alternative chemistries; therefore, there is an increasing need for novel chemical control options. Adult feeding, a strategy used with insecticide-treated baits for other fly species, has yet to be tested as an option for a chemical control delivery for cabbage maggot. Treated bait can exploit the feeding behavior of CM and expose them to insecticides in a field setting. In this study, the efficacy of 5 organic and 5 conventional insecticides was compared in laboratory bioassays of treated bait stations in Aurora, Oregon, USA. The mortality of adult female cabbage maggot flies was assessed over time following ingestion of insecticides. Among organic insecticides tested, spinosad was highly effective 4 h after exposure, while pyrethrins + azadirachtin was moderately effective following 18 h after exposure. Flies exposed to conventional-use pesticides zeta-cypermethrin and bifenthrin had high mortality 1.75 h after exposure, while spinetoram had moderate efficacy 2 h after exposure. Insecticides identified with high or moderate efficacy may have the potential for use in baits or lure formulations that could be used to augment the control of cabbage maggots in field settings.

The alfalfa weevil (Hypera postica Gyllenhal (Coleoptera: Curculionidae)), a key pest of alfalfa (Medicago sativa L. (Fabales: Fabacae)) across the US, has developed resistance to pyrethroids lambda-cyhalothrin and zeta-cypermethrin in at least 6 western US states. Unfortunately, 6 pyrethroid active ingredients represent most commercial insecticides registered for alfalfa weevil control in forage alfalfa systems. Thus, the loss of efficacy of this mode of action group due to multiple resistance represents a significant agricultural challenge because of a limited registered alternative mode of actions. To evaluate the extent and severity of resistance among pyrethroids around the United States, laboratory bioassays using larvae from Arizona, California, Montana, Oregon, Washington, and Wyoming, including both the Egyptian and western strains, were conducted. Results indicated that similar degrees of resistance among type II pyrethroids as determined by both laboratory bioassays and field trials exist. The LC50 values of alpha-cypermethrin, beta-cyfluthrin and zeta-cypermethrin produced significant correlations with the LC50 values of lambda-cyhalothrin. In contrast, resistance did not include type I pyrethroid, bifenthrin (registered for seed alfalfa production), whose LC50 values yielded a slope not significantly different from zero when correlated with lambda-cyhalothrin. Field trials conducted in Arizona, Montana, and Washington corroborated laboratory results, as commercial formulations with type II pyrethroid active ingredients failed to adequately control alfalfa weevils resistant to lambda-cyhalothrin. Integrated resistance management recommendations are discussed.

Due to rapidly developed resistance, pest management relies less on pyrethroids to control economically damaging infestations of the tarnished plant bug (TPB), Lygus lineolaris (Palisot de Beauvois) in cotton fields of Mississippi. Yet, pyrethroid resistance remains prevalent in TPB populations. This study assessed the resistance levels in adult TPB to six common pyrethroids and acephate. Resistant TBPs were collected from wild host plants in late October after harvest in the Mississippi Delta region of the United States. Based on LC values, the field-resistant TPBs displayed higher resistance to permethrin, esfenvalerate, and bifenthrin (approximately 30 fold) and moderate resistance to λ-cyhalothrin, β-cyfluthrin, ζ-cypermethrin, and acephate (approximately 15 fold). Further investigations showed that the inhibitors of three detoxification enzyme, triphenyl phosphate (TPP), diethyl maleate (DEM), and piperonyl butoxide (PBO) had synergistic effects on permethrin, λ-cyhalothrin, and bifenthrin in resistant TPBs. Furthermore, elevated esterase, GST, and P450 activities were significantly expressed in field-resistant TPBs. Additionally, GST and esterase were reduced after 48 h exposure to certain pyrethroids at LC dose. The synergistic and biochemical assays consistently indicated that P450 and esterase were involved in pyrethroid detoxification in TPBs. This study provides valuable information for the continued use of pyrethroids and acephate in controlling TPBs in cotton fields in the Mississippi Delta region of the United States.

The expansion of monocultures to regions close to conservation areas has put biodiversity at risk, mainly due to the intense use of pesticides. Anurans are highly susceptible to pesticides and may be a biological marker in the contamination of an area. However, methods for determining pesticides in anurans are incipient. In this work, a miniaturized QuEChERS method was developed for the extraction of atrazine, chlorpyrifos, α- and β-endosulfan, α-, β-, θ- and ζ-cypermethrin in anuran adipose tissues. The method was optimized for the tissue sample size scale according to sample mass availability. Extracting solvent and adsorbents for the clean-up step was evaluated, achieving recoveries next to 100% with acetonitrile and without a clean-up step. The mini-QuEChERS method, using 500 mg of adipose tissue, 50 mg of NaCl and 200 mg of MgSO, 100 μL of ultrapure water, and 1.50 mL of acetonitrile with no purification step, followed by high-performance liquid chromatography analysis and photodiode array detection was validated following the European Community guidelines. The methodology showed a moderate matrix effect for some pesticides, which was corrected using the matrix-matched calibration. The limits of quantification for the pesticide residues in adipose tissues ranged from 10 to 75 μg kg. Pesticide recoveries ranged from 74% to 115%, and repeatability and within-lab reproducibility showed relative standard deviations < 11%. The mini-QuEChERS method was applied to extract pesticide residues from the adipose tissues of two species of anurans: and . 25% of samples were positive, detecting endosulfan and chlorpyriphos, confirmed by liquid chromatography coupled to tandem mass spectrometry. The mini-QuEChERS was a simple, economical, and eco-friendly method for extracting pesticide residues in anuran adipose tissue samples.

Commercial beekeepers transporting honey bees across the United States to provide almond pollination services have reported honey bee deaths, possibly due to pesticide applications made during crop bloom. Pesticides are often applied as "tank mixes", or mixtures of fungicides and insecticides combined into a single application. Spray adjuvants are often added to tank mixes to improve the application characteristics of a pesticide and include spreaders, stickers, or surfactants. The goal of this research was to determine toxicity of adjuvants to adult worker honey bees, both when applied alone and in adjuvant-pesticide tank mixtures. Field-relevant combinations of formulated products were applied to 3-day-old adult worker honey bees using a Potter Spray Tower, and mortality was assessed 48 h following exposure. Adjuvants tested included Activator-90, Attach, Choice Weather Master, Cohere, Dyne-Amic, Induce, Kinetic, LI 700, Liberate, Nu-Film P, PHT Latron B-1956, and Surf-90; fungicides tested include Luna Sensation (Fluopyram and Trifloxystrobin), Pristine (Pyraclostrobin and Boscalid), Tilt (Propiconazole), and Vangard (Cyprodinil), and insecticides tested include Altacor (Chlorantraniliprole), Intrepid 2F (Methoxyfenozide), and a positive control Mustang Maxx (Zeta-cypermethrin). Results demonstrated that exposure to some adjuvants causes acute honey bee mortality at near-field application rates, both when applied alone and in combination with pesticides. Some adjuvant-pesticide combinations demonstrated increased toxicity compared with the adjuvant alone, while others demonstrated decreased toxicity. A better understanding of adjuvant and adjuvant-pesticide tank mixture toxicity to honey bees will play a key role in informing "Best Management Practices" for pesticide applicators using spray adjuvants during bloom when honey bee exposure is likely.

Drosophila suzukii Matsumura (Diptera: Drosophilidae) is a key pest of soft-skinned fruit such as blackberry and blueberry. Differing seasonal spray regimes are expected to have variable effects on D. suzukii populations. Semi-field cage trials were performed at three locations in the United States (Georgia, Oregon, and North Carolina) on blueberry and blackberry crops to evaluate this hypothesis. Insecticides with different efficacy rates (ZC - zeta-cypermethrin, SPI - spinetoram, CYAN - cyantraniliprole) were applied during field experiments conducted within large cages. Treatment schedules consisted of two insecticide applications which performed over three weeks. Seasonal treatment schedules were applied in the following order: ZC-CYAN and CYAN-ZC in rabbiteye and highbush blueberry with the addition of a ZC-SPI treatment applied in blackberry. In addition, a population model was applied to simulate the relative efficacy of the insecticide schedules in Oregon on D. suzukii population model based on previously published efficacy, biological, and weather parameters. Overall, all schedules resulted in reduced D. suzukii infestation compared to untreated control (UTC) treatments, with statistical differences in all three locations. The numerically lower infestation was found in some cases in ZC-CYAN schedule. Population modeling conducted exclusively for blueberry, and the simulations indicated no discernible differences between the two respective schedules (ZC-CYAN vs CYAN-ZC). The present study demonstrates that seasonal infestation of D. suzukii could be suppressed irrespective of application order. Additional research is required to assess the optimal timing and sequence of insecticide applications for controlling seasonal populations of D. suzukii in fruit crops. Such information could be invaluable for growers who are seeking to strategize their insecticide applications.

(Koch) (Acari: Tetranychidae), commonly known as European red mite, is a polyphagous pest of various tree and small fruit crops, including apples. A field study was conducted to evaluate different pesticide options available for the management of , and their impact on the population of non-target predatory mite species complex consisting of , , and in apple orchards. Pesticides were applied using a commercial airblast sprayer at the 3-5 mite/leaf recommended economic Integrated Pest Management (IPM) threshold or prophylactically in the spring ignoring IPM practices such as monitoring, reliance on biological control and economic thresholds. Effects on the motile and egg stages of were evaluated as were effects on the populations of predatory mites through frequent leaf counts during the season. We also recorded the subsequent overwintering eggs of from each pesticide treatment. The two prophylactic treatments containing a mixture of zeta-cypermethrin + avermectin B1 + 1% horticultural oil and abamectin + 1% horticultural oil provided effective control of population throughout the season without reducing predatory mite populations. In contrast, eight treatments applied at the recommended economic threshold of 3-5 mites/leaf were not effective in suppressing populations and most reduced predatory mites. Etoxazole had significantly higher number of overwintering eggs compared to all other treatments.

According to Article 12 of Regulation (EC) No 396/2005, EFSA has reviewed the maximum residue levels (MRLs) currently established at European level for the group of pesticide active substances cypermethrins. To assess the occurrence of residues in plants, processed commodities, rotational crops and livestock, EFSA considered the conclusions derived in the framework of Regulation (EC) No 1107/2009, the MRLs established by the Codex Alimentarius Commission (codex maximum residue limits; CXLs) for and as well as the European authorisations and import tolerances reported by Member States and the UK (including the supporting residues data) for . The toxicological profile of was also assessed. Based on the assessment of the available data, MRL proposals were derived, and a consumer risk assessment was carried out. Some information required by the regulatory framework was missing and a possible chronic/acute risk to consumer was identified. Hence, the consumer risk assessment is considered indicative only, all MRL proposals derived by EFSA still require further consideration by risk managers and measures for reduction of the consumer exposure should also be considered.

Even though honey bees in the field are routinely exposed to a complex mixture of many different agrochemicals, few studies have surveyed toxic effects of pesticide mixtures on bees. To elucidate the interactive actions of pesticides on crop pollinators, we determined the individual and joint toxicities of thiamethoxam (THI) and other seven pesticides [dimethoate (DIM), methomyl (MET), zeta-cypermethrin (ZCY), cyfluthrin (CYF), permethrin (PER), esfenvalerate (ESF) and tetraconazole (TET)] to honey bees (Aplis mellifera) with feeding toxicity test. Results from the 7-days toxicity test implied that THI elicited the highest toxicity with a LC data of 0.25 (0.20-0.29) μg mL, followed by MET and DIM with LC data of 4.19 (3.58-4.88) and 5.30 (4.65-6.03) μg mL, respectively. By comparison, pyrethroids and TET possessed relatively low toxicities with their LC data from the range of 33.78 (29.12-38.39) to 1125 (922.4-1,442) μg mL. Among 98 evaluated THI-containing binary to octonary mixtures, 29.59% of combinations exhibited synergistic effects. In contrast, 18.37% of combinations exhibited antagonistic effects on A. mellifera. Moreover, 54.8% pesticide combinations incorporating THI and TET displayed synergistic toxicities to the insects. Our findings emphasized that the coexistence of several pesticides might induce enhanced toxicity to honey bees. Overall, our results afforded worthful toxicological information on the combined actions of neonicotinoids and current-use pesticides on honey bees, which could accelerate farther comprehend on the possible detriments of other pesticide mixtures in agro-environment.