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Extrafloral nectar from coffee-associated trees as alternative food for a predatory mite.
- Date:
- Author: Iasczczaki RS  |  Pallini A  |  Venzon M  |  Beghelli GM  |  de Assis CB  |  Marcossi I  |  Janssen A  | 
Plant diversity can enhance natural pest control in agriculture by providing resources and conditions that are not regularly available in conventional crops to natural enemies of crop pests. Extrafloral nectar-producing plants, for example, might cause reduction of pest densities on neighboring plants because the nectar can increase the performance of natural enemies. Coffee agroforestry systems often contain extrafloral-nectar-producing Inga spp. trees that serve several purposes. Recent studies suggest that they attract and arrest a diversity of natural enemies that contribute to the control of coffee pests. Mites from the Phytoseiid family are key natural enemies of coffee pest mites, but no study has investigated whether Inga extrafloral nectar increases the performance of predatory mites in coffee ecosystems. Thus, here, we assessed whether the extrafloral nectar of Inga edulis Mart. (Fabaceae) can be considered a suitable nutritional resource for the predatory mite Amblyseius herbicolus (Chant), one of the most abundant phytoseiids in coffee crops. We found that feeding on extrafloral nectar allows for development and survival, but not reproduction, of A. herbicolus. Whereas individuals that fed on a diet of nectar during their immature development could subsequently only oviposit after having fed on a pollen diet, individuals that had developed on pollen stopped ovipositing when fed nectar. Our findings suggest that interplanted Inga trees can help to conserve populations of predatory mites in crop ecosystems through the provision of nectar and may boost biological control services. Future research should investigate the effects of extrafloral nectar-producing trees on coffee pest control by these predatory mites.
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Economic growth and health awareness spotlight opportunities and challenges in the food industry, particularly with decreasing arable land, climate change, dwindling freshwater resources, and pollution affecting traditional protein sources. Microalgae have emerged as a promising alternative, with higher protein content, better nutritional quality, and greater environmental resilience compared to conventional crops. They offer a protein balance comparable to meat, making them a sustainable protein source with health benefits like antioxidants, cardiovascular support, and anti-inflammatory properties. Improving the protein content of microalgae through optimized cultivation techniques is crucial to fully realize its potential as a novel food source. While there are already microalgae-based food products in the market, challenges remain in utilizing microalgal protein for widespread food production, emphasizing the need for further research. This review article explores the impact of microalgae culture conditions on protein content, the physicochemical and nutritional characteristics of microalgal protein, the health advantages of microalgal proteins and their derivatives, as well as research on separating and purifying microalgal proteins and their derivatives. It also delves into the current opportunities and obstacles of microalgal proteins and their derivatives as food, highlighting the potential for investigating the link between microalgal protein food and human health.
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Duckweed protein: Extraction, modification, and potential application.
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- Author: Sulaiman NS  |  Mohd Zaini H  |  Wan Ishak WR  |  Matanjun P  |  George R  |  Mantihal S  |  Ching FF  |  Pindi W  | 
Discovering alternative protein sources that are both nutritious and environmentally friendly is essential to meet the growing global population's needs. Duckweed offers promise due to its cosmopolitan distribution, rapid growth, high protein content, and scalability from household tanks to large lagoons without requiring arable land that competes for the major crops. Rich in essential amino acids, particularly branched-chain amino acids, duckweed supports human health. Extraction methods, such as ultrasound and enzymatic techniques, enhance protein yield compared to traditional methods. However, low protein solubility remains a challenge, addressed by protein modification techniques (physical, chemical, and biological) to broaden its applications. Duckweed proteins hold potential as functional food ingredients due to their unique physicochemical properties. This review also includes patents and regulations related to duckweed protein, filling a gap in current literature. Overall, duckweed presents a sustainable protein source with a lower environmental impact compared to conventional crops.
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Exploring different processes for starch extraction from microalgae and synthesis of starch-chitosan plastic films.
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- Author: Di Caprio F  |  Pedram N  |  Brugnoli B  |  Francolini I  |  Altimari P  |  Pagnanelli F  | 
Microalgae could become a more sustainable starch source than conventional crops. However, available refinery processes are lacking. In this study, we develop different innovative processes to refine microalgal starch and obtaining starch-based bioplastics. After lipid extraction, defatted microalgae were treated by different routes: enzymatic treatment with Alcalase; sonication in hot water or dimethyl sulfoxide (DMSO) followed by precipitation with ethanol. Enzymes allows to extract 70 % of proteins while recovering 75 % of the initial starch in the residual pellet, with a purity of 58 %. The most effective configuration based on sonication and water/DMSO extraction allowed to recover up to 80 % starch with 80-91 % purity. Chitosan improved the mechanical properties of the obtained starch-based films. The use of defatted algae or purified starch gave different properties to the films (as rigidity and water stability) showing the possibility to tailor the material characteristics depending on the biorefinery route applied.
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HASCH - A high-throughput amplicon-based SNP-platform for medicinal cannabis and industrial hemp genotyping applications.
- Date:
- Author: Mansueto L  |  Tandayu E  |  Mieog J  |  Garcia-de Heer L  |  Das R  |  Burn A  |  Mauleon R  |  Kretzschmar T  | 
Cannabis sativa is seeing a global resurgence as a food, fiber and medicinal crop for industrial hemp and medicinal Cannabis industries respectively. However, a widespread moratorium on the use and research of C. sativa throughout most of the 20th century has seen the development of improved cultivars for specific end uses lag behind that of conventional crops. While C. sativa research and development has seen significant investments in the recent past, resulting in a suite of publicly available genomic resources and tools, a versatile and cost-effective mid-density genotyping platform for applied purposes in breeding and pre-breeding is lacking. Here we report on a first mid-density fixed-target SNP platform for C. sativa.
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Economic Injury Levels and Economic Thresholds for as a Function of Insecticide Application Technology in Organic and Conventional Coffee (), Farms.
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- Author: Picanço Filho MC  |  Lima E  |  Carmo DDGD  |  Pallini A  |  Walerius AH  |  da Silva RS  |  Sant'Ana LCDS  |  Lopes PHQ  |  Picanço MC  | 
(Lepidoptera: Lyonetiidae) is one of the main pests in coffee crops. The economic injury level (EIL) is the lowest density of the pest at which economic damages match the costs of control measures. The economic threshold (ET) is the density of the pest at which control measures must be taken so that this population does not reach the EIL. These are the main indices used for pest control decision-making. Control of is carried out by manual, tractor, airplane or drone applications. This work aimed to determine EILs and ETs for as a function of insecticide application technology in conventional and organic crops. Data were collected over five years in commercial crops on seven 100 ha central pivots. The cost of control in organic crops was 16.98% higher than conventional. The decreasing order of control cost was manual > drone > airplane > tractor application. Coffee plants were tolerant to low densities (up to 15% mined leaves) of the pest that caused losses of up to 6.56%. At high pest densities (54.20% mined leaves), losses were high (85.62%). In organic and conventional crops and with the use of different insecticide application technologies, EIL and ET were similar. The EIL and ET were 14% and 11% of mined leaves, respectively. Therefore, these indices can be incorporated in integrated pest management programs in crops. The indices determined as a function of insecticide application technology in organic and conventional coffee are important as they serve producers with different technological levels. Additionally, EILs and ETs can contribute to more sustainable production, as control methods will only be employed when the pest density reaches these indices.
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Valorization of Duval-Jouve Young Shoots in Healthy and Sustainable Diets.
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- Author: Sánchez Gavilán I  |  Velázquez Ybarzabal D  |  de la Fuente V  |  Cámara RM  |  Sánchez-Mata MC  |  Cámara M  | 
The revalorization of natural resources in food production is increasing, and the effect of climate change is negatively affecting the production of conventional crops. In recent years, edible halophytes have received more attention due to their ability to tolerate a wide range of salinities. Thus, the use of halophytes that require less water and are strongly adapted to high-salinity soil and coastal areas can provide sustainable agriculture in certain areas. In addition, there is growing interest in the study of the possibilities that these species offer as foods due to their excellent nutritional profile and antioxidant properties. For that reason, the exploitation of plants adapted to these areas is nowadays even more important than in the past to guarantee food security in arid or semiarid salinized territories. The available data about the nutrients and bioactive compounds composition of many non-cultivated edible vegetables traditionally used in the Mediterranean area, such as Salicornia edible young shoots, are still scarce. With the aim of improving the knowledge on their nutritional value, the present study provides new data about the content of some compounds with biological activity, such as fiber and organic acids, in eight samples of young shoots of Duval-Jouve gathered in great mainland and coastal salt marshes in Southwest and Central Spain. Results showed that this vegetable can be considered a healthy food and a very good source of dietary fiber (4.81-6.30 g/100 g fw total fiber). Its organic acid profile showed oxalic, malic, citric and succinic acids. Oxalic acid was the major one, with mean values of 0.151-1.691 g/100 g fw. From the results obtained in this study, shoots could be recommended as an alternative source of fiber for healthy and sustainable diets in the general adult population with no risk of renal disease.
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Enhancing the bio-prospective of microalgae by different light systems and photoperiods.
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- Author: Parveen A  |  Bhatnagar P  |  Gautam P  |  Bisht B  |  Nanda M  |  Kumar S  |  Vlaskin MS  |  Kumar V  | 
Microalgae are a source of highly valuable bioactive metabolites and a high-potential feedstock for environmentally friendly and sustainable biofuel production. Recent research has shown that microalgae benefit the environment using less water than conventional crops while increasing oxygen production and lowering CO emissions. Microalgae are an excellent source of value-added compounds, such as proteins, pigments, lipids, and polysaccharides, as well as a high-potential feedstock for environmentally friendly and sustainable biofuel production. Various factors, such as nutrient concentration, temperature, light, pH, and cultivation method, effect the biomass cultivation and accumulation of high-value-added compounds in microalgae. Among the aforementioned factors, light is a key and essential factor for microalgae growth. Since photoautotrophic microalgae rely on light to absorb energy and transform it into chemical energy, light has a significant impact on algal growth. During micro-algal culture, spectral quality may be tailored to improve biomass composition for use in downstream bio-refineries and boost production. The light regime, which includes changes in intensity and photoperiod, has an impact on the growth and metabolic composition of microalgae. In this review, we investigate the effects of red, blue, and UV light wavelengths, different photoperiod, and different lighting systems on micro-algal growth and their valuable compounds. It also focuses on different micro-algal growth, photosynthesis systems, cultivation methods, and current market shares.
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In order to effectively address the issue of severe soil salinization in the coastal area of the Yellow River Delta, which has led to a significant number of medium and low-yield fields in this region, and to satisfy the rising demand for feed grain in China in recent years, a highly effective solution is to replace conventional crops by cultivating a specialized type of forage grass that can withstand high salinity levels and is well adapted to the local climate. This study proposed a spatial layout scheme for planting salt-tolerant forages, with the aim of providing a foundation for enhancing saline-alkali land and increasing resource utilization efficiency. The results showed that the climate conditions in the Yellow River Delta were suitable for planting sweet sorghum. With respect to soil salt content, the suitable planting regions for sweet sorghum can be classified into four categories: Suitable, moderately suitable, less suitable, and unsuitable, with soil salt concentrations of 2.62-5.25‱, 5.25-7.88‱, respectively. Concerning economic benefits, sweet sorghum's input-output ratio (74.4%) surpasses that of cotton in high saline-alkali zones, providing a significant advantage in comparison with traditional crops. In non-saline-alkali and light saline-alkali areas, the traditional winter wheat-summer maize planting system offers higher economic benefits and nitrogen use efficiency, so it is recommended to maintain this system as the dominant agricultural model. In moderately and severe saline-alkali zones, although one-season maize exhibits greater nitrogen efficiency, its economic benefits are lower than those of sweet sorghum. Hence, it is advisable to promote one-season maize in suitable regions and introduce salt-tolerant forage, such as sweet sorghum in other areas. This approach offers novel ideas and methods for crop spatial layout planning and addresses potential feed grain shortages in the region.
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Gastrointestinal Microbial Ecology of Weaned Piglets Fed Diets with Different Levels of Glyphosate.
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- Author: Rani S  |  Sørensen MT  |  Estellé J  |  Noel SJ  |  Nørskov N  |  Krogh U  |  Foldager L  |  Højberg O  | 
Glyphosate possesses antimicrobial properties, and the present study investigated potential effects of feed glyphosate on piglet gastrointestinal microbial ecology. Weaned piglets were allocated to four diets (glyphosate contents [mg/kg feed]: 0 mg/kg control [CON; i.e., basal diet with no glyphosate added], 20 mg/kg as Glyphomax commercial herbicide [GM], and 20 mg/kg [IPA] and 200 mg/kg [IPA] as glyphosate isopropylamine [IPA] salt). Piglets were sacrificed after 9 and 35 days of treatment, and stomach, small intestine, cecum, and colon digesta were analyzed for glyphosate, aminomethylphosphonic acid (AMPA), organic acids, pH, dry matter content, and microbiota composition. Digesta glyphosate contents reflected dietary levels (on day 35, 0.17, 16.2, 20.5, and 207.5 mg/kg colon digesta, respectively). Overall, we observed no significant glyphosate-associated effects on digesta pH, dry matter content, and-with few exceptions-organic acid levels. On day 9, only minor gut microbiota changes were observed. On day 35, we observed a significant glyphosate-associated decrease in species richness (CON, 462; IPA, 417) and in the relative abundance of certain genera: CF231 (CON, 3.71%; IPA, 2.33%; IPA, 2.07%) and g_0.24 (CON, 3.69%; IPA, 2.07%; IPA, 1.75%) in cecum. No significant changes were observed at the phylum level. In the colon, we observed a significant glyphosate-associated increase in the relative abundance of (CON, 57.7%; IPA, 69.4%; IPA, 66.1%) and a decrease in (CON, 32.6%; IPA, 23.5%). Significant changes were only observed for few genera, e.g., g_0.24 (CON, 7.12%; IPA, 4.59%; IPA, 4.00%). In conclusion, exposing weaned piglets to glyphosate-amended feed did not affect gastrointestinal microbial ecology to a degree that was considered actual dysbiosis, e.g., no potential pathogen bloom was observed. Glyphosate residues can be found in feed made from genetically modified glyphosate-resistant crops treated with glyphosate or from conventional crops, desiccated with glyphosate before harvest. If these residues affect the gut microbiota to an extent that is unfavorable to livestock health and productivity, the widespread use of glyphosate on feed crops may need to be reconsidered. Few studies have been conducted to investigate potential impact of glyphosate on the gut microbial ecology and derived health issues of animals, in particular livestock, when exposed to dietary glyphosate residues. The aim of the present study was therefore to investigate potential effects on the gastrointestinal microbial ecology of newly weaned piglets fed glyphosate-amended diets. Piglets did not develop actual gut dysbiosis when fed diets, containing a commercial herbicide formulation or a glyphosate salt at the maximum residue level, defined by the European Union for common feed crops, or at a 10-fold-higher level.
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