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The school foodservice is a facility that offers catering services to students and exerts a significant influence on their well-being and academic performance. Hence, it is crucial to comprehend the present global research status and development trends of school foodservice over the last decade, investigate their future direction of progress and enhancement strategies, offer guidance to school canteen managers and policymakers, and foster the wholesome advancement of school foodservice.
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Catalytic Application of Biochar Functionalized Copper-l-histidine for the Chemo and Homoselective Conversion of Cyanides to Amides and Reduction of Nitroarenes to anilines.
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- Author: Farrokh M  |  Hajjami M  |  Zolfigol MA  |  Jalali-Mola S  | 
In our study, we aimed to use olive pomace, food industry waste, as biomass to produce biochar nanoparticles. The surface of the biochar was functionalized with the l-histidine ligand, and then cupric acetate was added to prepare Cu-l-histidine@biochar as a final catalyst for the chemo- and homoselective synthesis of amide and aniline derivatives. To characterize the novel catalyst, we employed various techniques. Another notable feature of this catalyst is its reusability, which maintained significant efficiency even after multiple uses in reactions.
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Creating Single-Cell Protein-Producing Mutants Using Chemical Mutagen and Amino Acid Inhibitors.
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- Author: Berzina I  |  Kalnins M  |  Geiba Z  |  Raita S  |  Palcevska J  |  Mika T  |  Spalvins K  | 
Due to population growth and climate changes, there is a rising need for alternative food and protein sources to reduce protein scarcity and the environmental impact of food industries. Single-cell proteins (SCPs) have the potential to partially or fully substitute plant- and animal-derived dietary proteins. is an appealing bacterium for SCP production because of its fast growth and ability to obtain high protein and essential amino acid (AA) content in its biomass. It is also capable of utilizing a wide range of substrates. attractiveness and efficiency can be further enhanced using mutagenesis. In this study, a novel approach to creating mutant strains with enhanced protein and AA content was experimentally validated. The method is based on the application of AA inhibitors for selective pressure to ensure the growth of mutants with enhanced protein and/or AA synthesis capacity. For AA inhibitors, three herbicides were used: glufosinate-ammonium (GA), L-methionine sulfoximine (MSO), and S-(2-aminoethyl)-L-cysteine (AEC). Initially, AA inhibitor doses for the complete inhibition of wild-type (WT) strain were determined. Then, was treated with EMS chemical mutagen and created mutants were cultivated on a medium containing inhibitory dose of AA inhibitors. Growing samples were selected, analyzed, and compared. The optimal inhibitory concentrations of herbicides for mutant selection were 0.05-0.4 M for GA, 0.01-0.05 M for MSO, and 0.2 M for AEC. The best-performing mutants were selected when using GA-improvement of 7.1 times higher biomass content, 1.5 times higher protein concentration, 1.2 times higher AA content, and 1.2 times higher essential AA index was achieved in comparison with WT . Enhanced mutants were also successfully selected when using MSO and AEC. This study demonstrates the potential of using AA inhibitors for the selection of mutants with improved protein and AA profiles.
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Eco-friendly synthesis of Carbon Quantum Dots (CQDs) from hazelnut husk for sensitive Aflatoxin B1 (AFB1) detection.
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- Author: Yuncu H  |  Nadaroglu H  |  Bozkurt E  | 
In this study, green fluorescent carbon quantum dots (CQDs) with remarkable stability, water solubility, and biocompatibility were synthesized from hazelnut husk (HH) waste material using a novel approach by the pyrolysis method. The optical properties of the synthesized HH-CQDs were characterized by UV-Vis and fluorescence spectroscopy (PL), while their structural properties were characterized using various techniques, including transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). TEM images revealed that HH-CQDs had a spherical shape with diameters ranging from 2 to 10 nm. The fluorescence quantum yield of these CQDs was measured as 0.04. Furthermore, CQDs were very effective at finding aflatoxin B1 (AFB1) using a fluorescence resonance energy transfer (FRET) mechanism, with a clear fluorescence emission peak seen at 451 nm. The photoluminescent properties of CQDs were evaluated under various pH conditions, showing a blue shift and increased fluorescence intensity at pH 9-10, suggesting their potential use in pH-sensitive sensor applications. This study demonstrates the selective and sensitive detection of AFB1 using HH-CQDs, with a strong linear relationship (R² = 0.9936) between fluorescence intensity and AFB1 concentration in the range of 25-250 ppm, and high accuracy in real food samples, including 81.56 % in corn, 98.64 % in milk, and 95.73 % in peanuts. This eco-friendly and cost-effective synthesis method offers a promising alternative for AFB1 detection in food samples by utilizing waste material to create valuable analytical tools.
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Mechanisation applied to entomological production cannot ignore insect reactivity: a case study on in the context of the 'Serinnovation' project.
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- Author: Giora D  |  Assirelli A  |  Cappellozza S  |  Saviane A  |  Sartori L  |  Dalla Montà A  |  Paglia G  |  Pavanello C  |  Fila G  |  Marinello F  | 
In December 2017 the Venetian Region (local Authority), financed the creation of the Operational Group (OG) 'Serinnovation', within the framework of the Rural Development Plan supported by the European Community. The OG aims at coordinating and spreading innovation in sericulture through mechanisation of processes and centralisation of some rearing steps, the use of waste as by-products and traceability to promote local productions. The project acts on perceived quality by increasing the added value, through production cost efficiency, and on the recovery of the waste material for further applications (circular economy). The final target was to develop a niche-process to obtain traceable 'Made-in-Italy' silk for the luxury market and non-textile applications. A first strategy to increase the efficiency of the process was to build an automatic leaf cutting machine to prepare the feed for the first three instars of the silkworm ( Linnaeus). This new machine - based on a patent - was validated through several tests and compared with the cutting system previously used. The study was completed by a bioassay of production and survival rate associated with the introduction of this innovation. The results showed that labour saving is in the order of 10% compared to a semi-manual process, the leaf quality is not affected, survival of larvae and silk production are not significantly different from the control. This methodology is proposed as a study case for other similar mechanisation processes in entomological production, as the impact of innovations on insect physiology should be carefully considered.
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Evaluating sorbents for reducing per- and polyfluoroalkyl substance mobility in biosolids-amended soil columns.
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- Author: Openiyi EO  |  Lee LS  |  Alukkal CR  | 
Sustainable reuse of biosolids as fertilizers is being threatened by the presence of per- and polyfluoroalkyl substances (PFAS) in our waste stream warranting research on strategies that will minimize PFAS mobility from land-applied biosolids. Here, we evaluated the ability of waste-derived sorbents aluminum chlorohydrate water treatment residuals (ACH-WTRs, 1 wt%) and biosolids-based biochar (1.5 wt%) to reduce mobility of PFAS in columns with 3 wt% biosolids-amended soils with and without sorbent layered on top of soil only and operated under transient unsaturated conditions. Cycles of simulated rain events of approximately three pore volumes distributed over a 4-day period followed by 3 days of drying were imposed for 6 months. Total PFAS concentrations in collected leachates were lower in the sorbent-treated columns compared to the control columns. Biochar outperformed the ACH-WTR with 41% versus 32% lower total PFAS in leachate, respectively, compared to the control. The most significant mitigation effect was observed with PFOS (perfluorooctane sulfonate) with 68% and 62% less PFOS in the leachates from the columns treated with ACH-WTR or biochar compared to the control, respectively. These results provide a first-of-its-kind assessment of the potential benefit of co-applying WTRs or biochar with biosolids to reduce PFAS mobility in biosolids-amended soils.
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Assessment of type and quantities of food and beverage plastic packaging: A case study.
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- Author: Bošković G  |  Stanisavljević N  |  Batinić B  |  Kovačević S  |  Jovičić N  |  Cvetanović A  | 
Plastic pollution has been identified as one of the most pressing environmental issues of the 21st century, driven by excessive consumption and inadequate plastic waste management. This issue is particularly reflected in short lifespan of plastic products, particularly plastic packaging within the food and beverage (F&B) industry. Urgent and relevant policy actions are needed to promote plastic circularity and improve waste management practices. Developing countries such as Montenegro face significant challenges in managing plastic waste flows due to legal, institutional and infrastructural gaps. This research addresses these challenges by employing material flow analysis (MFA) and advanced techniques like artificial neural networks to estimate the quantities and types of plastic packaging used in the F&B sector in Montenegro. The findings reveal that over 21,300 tonnes of F&B plastic packaging were placed on the market in Montenegro in 2018. Approximately 11% of this amount ends up directly littered in the environment or dumpsites, whereas the remaining 89% is predominantly collected and deposited in controlled landfills. Detailed MFA models were developed separately for the eight most common polymer types used in the F&B sector, along with specific models for plastic bags and polyethene terephthalate bottles to explore closed-loop recycling systems. It was found that only a small fraction of all analysed types of plastic packaging placed on the market can be effectively treated and recycled. This research contributes to the understanding of plastic waste management in Montenegro and facilitates the formulation of effective strategies to mitigate plastic pollution in the F&B industry.
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Solid-state fermentation of green waste for the production of biostimulants to enhance lettuce (Lactuca sativa L.) cultivation under water stress: closing the organic waste cycle.
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- Author: Solano Porras RC  |  Ghoreishi G  |  Sánchez A  |  Barrena R  |  Font X  |  Ballardo C  |  Artola A  | 
Food production faces important challenges such as water scarcity and the overall need of novel sustainable strategies. This study assesses the effect of the biostimulant produced through solid-state fermentation (SSF) of green waste (wood chips and grass residues) inoculated with Trichoderma harzianum with and without L-tryptophan as a precursor for indole-3-acetic acid (IAA) production, a well-known plant hormone. The fermented solid demonstrated significant positive effects on the growth of lettuce (Lactuca sativa L.) under different irrigation conditions. Substantial enhancements were observed in growth parameters such as fresh weight, plant height, leaf area and leaf quantity, along with chemical parameters including total phenol content, chlorophylls, carotenoids, and antioxidant activity (DPPH). The results also showed a positive impact on the nutritional quality of lettuce, particularly under normal irrigation conditions. In conclusion, this study highlights the biostimulant potential to improve the yield and nutritional quality of lettuce crops by reusing plant residues. Additionally, it poses the relevance of applying circular economy principles in sustainable agriculture and organic waste management.
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Ex situ fabrication and bioactivity characterization of Neem and Sage-infused bacterial cellulose membranes for sustainable antimicrobial applications.
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- Author: Fatima A  |  Ul-Islam M  |  Yasir S  |  Khan S  |  Manan S  |  Shehzad A  |  Ahmad MW  |  Al-Shannaq R  |  Islam SU  |  Abbas Y  |  Subhan F  |  Sabour AAA  |  Alshiekheid M  |  Ullah MW  | 
This study presents the ex situ development and characterization of bacterial cellulose (BC) membranes loaded with bioactive Sage and Neem extracts for enhanced antimicrobial applications. Utilizing discarded fruit waste as a cost-effective carbon source, BC production was optimized, yielding membranes with improved properties. Neem and Sage extracts, obtained via Soxhlet extraction, exhibited significant antibacterial activity against Escherichia coli and Staphylococcus aureus, with minimum inhibitory concentrations of 3.125 mg/mL and 25 mg/mL, respectively, for Neem extract, and 25 mg/mL and 50 mg/mL for Sage extract. These extracts (20 wt%) were successfully incorporated into BC membranes ex situ, resulting in BC-Neem (BC-N) and BC-Sage (BC-S) composites. Fourier-transform infrared spectroscopy (FTIR) confirmed the chemical interactions between the extracts and the BC matrix, revealing the introduction of new functional groups and enhancing the composite properties. Scanning electron microscopy (SEM) illustrated changes in morphology, indicating deeper penetration and attachment of the extracts within the BC structure. Quantitative analysis of water holding capacity demonstrated that BC-N and BC-S absorbed about 90 times water of their dry weight. Antibacterial assays through the colony-forming unit method showed that BC-N significantly inhibited S. aureus growth by 78 % and E. coli by 51 %, while BC-S exhibited a 48 % reduction against S. aureus. Agar disc-diffusion assay showed the formation of inhibition zones of 1.2 cm and 0.1 cm by BC-N against S. aureus and E. coli, respectively, in contrast to 0.2 cm and no inhibition by BC-S composite. These results highlight the potential of bioactive plant extract-loaded BC membranes as effective antimicrobial agents, offering a sustainable alternative to conventional materials in medical and food packaging applications.
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Enset starch-based biocomposite film reinforced with Ethiopian bentonite clay: Improved mechanical and barrier properties.
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- Author: Achenef HA  |  Emire SA  |  Kassahun SK  |  Kim H  | 
Improper disposal of traditional plastics leads to the generation of microplastics, resulting in severe pollution of land and oceans and posing a threat to human health and marine ecosystems. Hence, adopting eco-friendly bioplastics, particularly in food packaging, is essential. In this study, Enset starch-based biocomposite films, reinforced with Ethiopian bentonite clay at various ratios (0, 2.5, 5, 7.5 and 10 % w/w) were prepared using solvent casting method. The effect of bentonite clay on biocomposite films on structural, physicochemical, and morphological properties were analyzed. Characterization tests confirmed the even distribution of bentonite, strengthening of bonds, and enhancement of the biocomposite film properties. The biocomposite film with 5 wt% bentonite clay incorporation into enset starch exhibits optimal performance; maximum strength (increased by 132 %), less water solubility (reduction in 33 %), reduction in water vapor permeability (decreased by 42 %), and better compatibility in the morphologies attributed by the intercalated silicate layer. This study highlights the effectiveness of bentonite clay in enhancing enset starch biocomposite properties, offering a promising eco-friendly solution for biodegradable food packaging and promoting sustainable resource utilization.
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