Pork feet (PF) contaminated with Salmonella are often discarded by the food industry, because heat treatment is not economically viable for these products. Dry salting can be an alternative to heat treatment if it can significantly reduce Salmonella populations. The aim of this study was to assess the Salmonella´s survival in PF during dry salting process. Firstly, Salmonella was quantified in contaminated PF coming from the food industry. Thereafter, PF samples were artificially contaminated with three levels of Salmonella (3, 6 and 8 log CFU/g) and stored at 10°C for 30 days. Salmonella survival was assessed by counts on Xylose Lysine Deoxycholate Agar, Mannitol Lysine Crystal-Violet Brilliant-Green Agar and by the 3M™ 2 Molecular Detection Assay (MDA). After 18 days of salting, the 3 log CFU/g inoculum was no longer detected by the cultural and detection methods. The 6 and 8 log CFU/g inoculums were not detected on the agar plates after 26 days of salting but were still positive by MDA. Considering that Salmonella counts in contaminated PF in the food industry was 1.56 log CFU/g and that populations of 3 to 8 log CFU/g of Salmonella were reduced to unculturable levels after dry salting, this process could contribute to the control of Salmonella in PF.

(grass pea) is a valuable crop for sustainable agriculture, offering dietary benefits and desirable agronomic traits. However, its yield stability is limited by diseases such as powdery mildew caused by . Increasing fungal resistance to pesticides and environmental concerns demand the development of resistant crop varieties. To identify key defense mechanisms and effector genes involved in the - interaction we analyzed four accessions exhibiting varying resistance to (resistant, partially resistant, partially susceptible, and susceptible) using a dual RNA-Seq experiment across different time points. We observed a host biphasic response, characterized by an initial burst of gene expression, followed by a quiescent phase, and a subsequent wave of intense gene expression. Common defense mechanisms included antifungal protein expression, cell wall reinforcement, and reactive oxygen species-mediated defense. These defenses involved respectively Bowman-Birk type proteinase inhibitors, peptidyl-prolyl cis-trans isomerases and mannitol dehydrogenases. The resistant accession specifically activated early reinforcement of structural barriers associated with lignin biosynthesis and the phenylpropanoid pathway, along with sustained chemical defenses (e.g. ), epigenetic regulation, and oxidative stress responses thorough peroxidases and heat shock proteins. The partial resistant accession exhibited a front-loaded defense response at early infection stages. Contrastingly, the partially susceptible accession exhibited a weaker baseline defense, with a slower and less robust response targeting pathogen infection. We identified potential effectors, including genes involved in cell wall hydrolysis (e.g. mannosidase DCW1), nutrient acquisition (e.g. secreted alpha-glucosidase), and virulence (e.g. SnodProt1), with a higher diversity of effectors identified in the susceptible accession. In conclusion, this study identifies novel targets such as NLRs and effectors, antifungal proteins and genes related to cell wall reinforcement, within the complex - interaction to support future breeding programs aimed at enhancing resistance to in and related species.

Olive leaf, a by-product of the olive oil industry, is rich in bioactive compounds, including the antioxidant and anti-inflammatory oleuropein. Olive leaf extracts have been explored for nutraceutical applications, but oleuropein's low bioavailability and stability limit its use in food and supplements. This work aimed to mitigate these issues by nano-encapsulating the olive leaf extract in proniosomes-free-flowing powders that form niosomes upon hydration. These niosomes can then be further processed into dosage forms or incorporated into functional foods. Proniosomes based on lactose or mannitol were developed and characterized. Hydration of the proniosomes yielded niosomes with high oleuropein loading and antioxidant activity. These niosomes controlled oleuropein release in simulated gastric and intestinal fluids, protecting it from degradation. Furthermore, niosomal encapsulation enhanced protection against oxidative stress in intestinal cells compared to the unformulated extract, suggesting improved intracellular delivery and making this formulation a suitable candidate as a functional food ingredient.

Elevated intracranial pressure (ICP) is a critical condition associated with significant morbidity and mortality, requiring prompt and effective management. Mannitol and hypertonic saline (HTS) are the two most widely used hyperosmolar agents in clinical practice for ICP reduction, each with distinct pharmacologic properties, efficacy profiles, and safety considerations. This review aims to provide a comprehensive assessment of the mechanisms, clinical efficacy, safety, practical considerations, and guideline recommendations associated with the use of mannitol and HTS for the management of elevated ICP. Current available data does not clearly support one hyperosmolar agent over another and both agents are considered equivalent. Consensus recommendations vary, but the most recent recommendations seem to support the use of HTS over mannitol, mostly due to potential pharmacodynamic advantages that have been shown in smaller investigations. Further research is warranted to refine dosing strategies, clarify administration concerns, and address knowledge gaps in comparative efficacy and safety.

Overcoming the blood-brain barrier (BBB) remains a significant challenge for nucleic acid delivery to the brain. We have explored a combination of mannitol-modified poly (β-amino ester) (PBAE) nanoparticles and systemic mannitol injection for crossing the BBB. We incorporated mannitol in the PBAE polymer for caveolae targeting and selected monomers that may help avoid delivery to the liver. We also induced caveolae at the BBB through systemic mannitol injection in order to create an opportunity for the caveolae-targeting nanoparticles (M30 D90) containing plasmid DNA to cross the BBB. When a clinically relevant dose was administered intravenously in this caveolae induction model, M30 D90 demonstrated significant transgene expression of a reporter plasmid in the brain, with selective uptake by neuronal cells and minimal liver accumulation. We demonstrate that caveolae modulation using systemic mannitol administration and caveolae targeting using designed nanoparticles are necessary for efficient delivery to the brain. This delivery platform offers a simple, scalable, and controlled delivery solution and holds promise for treating brain diseases with functional targets.

To assess the technical feasibility and functional, metabolic, and structural myocardial integrity of the donor heart after four hours of direct coronary oxygen persufflation (COP).

Natural sweeteners have attracted widespread attention because they are eco-friendly, healthy, low in calories, and tasty. The demand for natural sweeteners is increasing together with the popularity of green, low-carbon, sustainable development. With the development of synthetic biology, microbial cell factories have emerged as an effective method to produce large amounts of natural sweeteners. This technology has significantly progressed in recent years. This review summarizes the pathways and the enzymes related to the biosynthesis of natural sweeteners, such as mogrosides, steviol glycosides, glycyrrhizin, glycyrrhetinic acid, phlorizin, trilobatin, erythritol, sorbitol, mannitol, thaumatin, monellin, and brazzein. Moreover, it focuses on the research about the microbial production of these natural sweeteners using synthetic biology methods, aiming to provide a reference for future research on the production of natural sweeteners.

The present work was aimed at studying the effect of different substrates on growth and biometabolite production in Cordyceps militaris fruiting body. Of the seven substrates tested alone and in combination with brown rice, it was observed that the combination of pearl millet and brown rice showed the highest yield of fruit body with biological efficiency of 104.3 ± 2.08%. Among the three major biometabolites (cordycepin, adenosine and D-mannitol), cordycepin was found to be highest [11.05 ± 0.03 mg/g dry weight (DW)] in fruit bodies grown on kidney bean. Adenosine was highest (3.30 ± 0.01 mg/g DW) in fruit bodies grown on pearl millet and D-mannitol content of 15.53 ± 0.23 mg/g DW was observed to be highest in fruit bodies obtained from substrate combination composed of cowpea and brown rice. The study highlights that choice of substrate significantly affects the yield and metabolite production of C. militaris.

Raspberry possesses several health benefits such as antitumor, antioxidant, and blood sugar-regulating activities. In order to improve the use value of raspberries, the optimal preparation process of raspberry chewable tablets (RCT) and its physical, chemical, antioxidant, and hypoglycemic properties were investigated in this research. The optimum formulation of RCT was determined as 39.1% raspberry powder, 13.1% red beet root powder, 9.8% corn starch, 15% microcrystalline cellulose, 14% mannitol, 1% magnesium stearate, and 8% ethanol (75%) by single-factor experiment and response surface methodology using sensory evaluation score and hardness as parameters. RCT prepared using the optimal formulation had a hardness of 47.33 ± 2.31 N, brittleness of 0.33%, weight of 1.00 ± 0.03 g, and a color difference ΔE = 0.427. The total phenolic and total flavonoid contents of RCT were 18.610 ± 0.483 mg/g and 3.885 ± 0.061 mg/g, respectively. Meanwhile, seven phenolic compounds in RCT were determined by high-performance liquid chromatography. Free radical scavenging abilities ((1,1-diphenyl-2-picrylhydrazyl, DPPH), (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), ABTS), and ·OH), ferric ion-reducing antioxidant power and cell tests proved the significant antioxidant ability of RCT. The obvious inhibitory activities of RCT on α-glucosidase and α-amylase reflected its hypoglycemic potential. This study provided an important basis for the development of raspberry functional foods with antioxidant and blood glucose regulating potential.

The recent pandemic has highlighted the urgent need to elucidate the pathophysiological mechanisms underlying viral effects in humans and is driving the search for innovative antiviral therapies. Several studies have investigated the ability of gas plasma, a partially ionized gas that simultaneously generates several reactive species, to be a new antiviral tool. However, several aspects of the mechanisms of antiviral action of gas plasma remained elusive. In this study, we, for the first time, used a gas plasma device approved for medical purposes and routinely applied in the clinics, especially for wound healing, to test its antiviral activity against a murine corona-virus in vitro (MHV-GFP), a research model analogous to human coronaviruses such as SARS-CoV-2. For this, we established a novel high-content imaging assay that gave quantitative and kinetic information about infection and reduced viral activity in murine fibroblasts (17Cl-1) host cells. Gas plasma treatment delayed viral infectivity and reduced overall infection and toxicity in 17Cl1 cells. Various antioxidants at different concentrations were screened to identify ROS relevant to antiviral effects. Catalase provided no virus protection, and DMSO, mannitol, histidine, Trolox, and ascorbic acid only modestly reduced gas plasma virucidal efficacy. By contrast, glutathione, tyrosine, and cysteine showed profound but not complete protection of MHV from gas plasma-derived reactive species, suggesting pivotal roles of superoxide radicals and peroxynitrite gas in plasma-driven viral inactivation. At extended gas plasma exposure times, fewer intact MHV RNA were detected, indicative of reactive species-driven RNA modifications or degradation as an additional mechanism of action. Virus particle size changes measured by electron microscopy were moderate. Collectively, we identified the potent antiviral activity of a clinically approved argon plasma jet along with potential mechanisms of action.